Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Sulphonamide derivatives with a triazine core as novel inducers of apoptosis and pyroptosis in glioblastoma multiforme cells

doi: 10.1080/14756366.2025.2594158

Figure Lengend Snippet: Results of LDH measurement of A172 (A) and U-87 MG (B) glioblastoma cells after 24 h of incubation with compounds. Means ± SD are shown with N = 3. ANOVA tests were used to demonstrate differences between cells treated with compounds and control. ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001.

Article Snippet: Human glioblastoma cell lines (A172 CRL-1620 TM , U-87 MG HTB-14 TM , U-118 MG HTB-15 TM , T98G CRL-1690 TM ) and the mouse astrocyte cell line (C8-D1A CRL-2541 TM ) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Incubation, Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Sulphonamide derivatives with a triazine core as novel inducers of apoptosis and pyroptosis in glioblastoma multiforme cells

doi: 10.1080/14756366.2025.2594158

Figure Lengend Snippet: Results of flow cytometry analysis of A172 (A) and U-87 MG (B) glioblastoma cells after 24 h of incubation with compounds. Means ± SD are shown with N = 4.

Article Snippet: Human glioblastoma cell lines (A172 CRL-1620 TM , U-87 MG HTB-14 TM , U-118 MG HTB-15 TM , T98G CRL-1690 TM ) and the mouse astrocyte cell line (C8-D1A CRL-2541 TM ) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Flow Cytometry, Incubation

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Sulphonamide derivatives with a triazine core as novel inducers of apoptosis and pyroptosis in glioblastoma multiforme cells

doi: 10.1080/14756366.2025.2594158

Figure Lengend Snippet: Results of flow cytometry analysis of A172 (A) and U-87 MG (B) glioblastoma cells after 24 h of incubation with compounds. Graphs visualise the cytograms from the compound analysis A172 (C) and U-87 MG (D). Means ± SD are shown with N = 4. ANOVA tests were used to demonstrate differences between cells treated with compounds and control. *** P ≤ 0.001; **** P ≤ 0.0001.

Article Snippet: Human glioblastoma cell lines (A172 CRL-1620 TM , U-87 MG HTB-14 TM , U-118 MG HTB-15 TM , T98G CRL-1690 TM ) and the mouse astrocyte cell line (C8-D1A CRL-2541 TM ) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Flow Cytometry, Incubation, Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Sulphonamide derivatives with a triazine core as novel inducers of apoptosis and pyroptosis in glioblastoma multiforme cells

doi: 10.1080/14756366.2025.2594158

Figure Lengend Snippet: Results of flow cytometry analysis of caspase 8 activation in A172 (A) and U-87 MG (B) glioblastoma cells after 24 h of incubation with compounds. Graphs visualise the cytograms from the compound analysis A172 (C) and U-87 MG (D). Means ± SD are shown with N = 4. ANOVA tests were used to demonstrate differences between cells treated with compounds and control. ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001.

Article Snippet: Human glioblastoma cell lines (A172 CRL-1620 TM , U-87 MG HTB-14 TM , U-118 MG HTB-15 TM , T98G CRL-1690 TM ) and the mouse astrocyte cell line (C8-D1A CRL-2541 TM ) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Flow Cytometry, Activation Assay, Incubation, Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Sulphonamide derivatives with a triazine core as novel inducers of apoptosis and pyroptosis in glioblastoma multiforme cells

doi: 10.1080/14756366.2025.2594158

Figure Lengend Snippet: Results of flow cytometry analysis of caspase 9 activation in A172 (A) and U-87 MG (B) glioblastoma cells after 24 h of incubation with compounds. Graphs visualise the cytograms from the compound analysis A172 (C) and U-87 MG (D). Means ± SD are shown with N = 4. ANOVA tests were used to demonstrate differences between cells treated with compounds and control. *** P ≤ 0.001; **** P ≤ 0.0001.

Article Snippet: Human glioblastoma cell lines (A172 CRL-1620 TM , U-87 MG HTB-14 TM , U-118 MG HTB-15 TM , T98G CRL-1690 TM ) and the mouse astrocyte cell line (C8-D1A CRL-2541 TM ) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Flow Cytometry, Activation Assay, Incubation, Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Sulphonamide derivatives with a triazine core as novel inducers of apoptosis and pyroptosis in glioblastoma multiforme cells

doi: 10.1080/14756366.2025.2594158

Figure Lengend Snippet: Results of flow cytometry analysis of caspase 3/7 activation in A172 (A) and U-87 MG (B) glioblastoma cells after 24 h of incubation with compounds. Graphs visualise the cytograms from the compound analysis A172 (C) and U-87 MG (D). Means ± SD are shown with N = 4. ANOVA tests were used to demonstrate differences between cells treated with compounds and control. * P ≤ 0.05; *** P ≤ 0.001; **** P ≤ 0.0001.

Article Snippet: Human glioblastoma cell lines (A172 CRL-1620 TM , U-87 MG HTB-14 TM , U-118 MG HTB-15 TM , T98G CRL-1690 TM ) and the mouse astrocyte cell line (C8-D1A CRL-2541 TM ) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Flow Cytometry, Activation Assay, Incubation, Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Sulphonamide derivatives with a triazine core as novel inducers of apoptosis and pyroptosis in glioblastoma multiforme cells

doi: 10.1080/14756366.2025.2594158

Figure Lengend Snippet: Results of H2DCFDA and DAPI double staining of glioblastoma cell lines after 30 min of compound treatment. Representative fluorescence microscope images are shown (A) - A172, and (C) U-87 MG cell lines. The graphs show the minimum, maximum and average fluorescence of each sample tested (B) - A172, and (D) U-87 MG. ANOVA tests were used to show differences between control and compound-treated cells. N > 100; ** P ≤ 0.01; **** P ≤ 0.0001.

Article Snippet: Human glioblastoma cell lines (A172 CRL-1620 TM , U-87 MG HTB-14 TM , U-118 MG HTB-15 TM , T98G CRL-1690 TM ) and the mouse astrocyte cell line (C8-D1A CRL-2541 TM ) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Double Staining, Fluorescence, Microscopy, Control

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Sulphonamide derivatives with a triazine core as novel inducers of apoptosis and pyroptosis in glioblastoma multiforme cells

doi: 10.1080/14756366.2025.2594158

Figure Lengend Snippet: Results of flow cytometry analysis of caspase 1 activation of A172 ( A ) and U-87 MG ( B ) glioblastoma cells after 24 h of incubation with compounds. Graphs visualise the cytograms from the compound analysis A172 ( C ) and U-87 MG ( D ). Means ± SD are shown with N = 4. ANOVA tests were used to demonstrate differences between cells treated with compounds and control. **** P ≤ 0.0001 ( E ) Confocal microscope images with colocalization of NF-κB (red) with the nucleus (DAPI, blue) under treatment of TMZ, MM118 and MM119 or not (control). In vivo evaluation of compounds MM118 and MM119. The confocal images were acquired using a 63x oil immersion objective, N = 3.

Article Snippet: Human glioblastoma cell lines (A172 CRL-1620 TM , U-87 MG HTB-14 TM , U-118 MG HTB-15 TM , T98G CRL-1690 TM ) and the mouse astrocyte cell line (C8-D1A CRL-2541 TM ) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Flow Cytometry, Activation Assay, Incubation, Control, Microscopy, In Vivo

Journal: Molecular Pharmaceutics

Article Title: Polymer–Temozolomide Conjugates as Therapeutics for Treating Glioblastoma

doi: 10.1021/acs.molpharmaceut.8b00766

Figure Lengend Snippet: Cell viability of polyMPC–TMZ random (a) and block (b) copolymers in U87MG (top) and T98G (bottom) glioblastoma cells; (c) IC 50 values for free TMZ and polyMPC–TMZ copolymers in both cell lines (± indicates standard deviation).

Article Snippet: Human glioblastoma (U87MG and T98G) cells were purchased from American Type Culture Collection (ATCC).

Techniques: Blocking Assay, Standard Deviation